ASTM D – 15 Standard Test Method for Analysis of Aqueous Extractable Protein in Latex, Natural Rubber, and Elastomeric Products Using the Modified. RRIM (MS P) and the ASTM (D ) modified Lowry tests, were examined and their relationship studied. Total extractable proteins, EPRRiMt. ASTM De1, Standard Specification for Rubber Surgical Gloves ASTM D, Standard Test Method for the Analysis of Aqueous.

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Protein levels are below 1, the lowest level of detection, as clean as a vinyl film negative control.

Water soluble proteins are extracted in a buffer solution and then precipitated to concentrate them and also to separate them from water soluble substances aatm may interfere with the determination. However, the 20 ppm proteins left, may happen to be harmful and the latex status as far as allergen content did not change at all.

ASTM D might be misleading and can cause unfortunate conclusions. Are you sure you want to empty the cart? It is the responsibility of the user of this standard to establish appropriate safety and health asgm and determine the applicability of regulatory limitations prior to use.

Remember me for one month. The extracted protein is redissolved and quantified colorimetrically by the modified Lowry method using a protein standard. However, traces still may remain and cause skin reactions.

ASTM-D, –

d5721 It is well known that proteins can transfer from wstm latex to the powder. Condoms with different lubricants as typically marketed, have not been tested in an ASTM ILS to determine if, and if so atsm what degree, the lubricant interferes with the assay.

Some of the proteins can be more harmful than others. Enter your personal account email address to request a password reset: November 20, Page last updated: A large number estimated in the order of magnitude of 10 percent in the U. Historical Version s – view previous versions of standard. This test method covers an analytical test for determining the amount of total aqueous extractable protein associated with NR, latex, and elastomeric products. Many efforts have been made to remove the proteins.

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Natural proteins are the main reason for Type I latex irritation. This paper suggests a way to eliminate the proteins with a simple, elegant and inexpensive method using fumed silica additives. Adkinson, Journal of Allergy and Clinical Immunology, 98, Experimental results and discussion Low-protein allergen analysis in latex compounded with fumed silica Compounding of fumed silica dispersion for dipped natural latex application resulted in tensile and tear enhancement astk described before.

Latex protein analysis ASTM D, might not represent a true picture of the latex irritation problem. Analytical information demonstrated that powdered latex gloves were found to contain high levels of protein between 1 and micrograms of protein per gram of rubber.

Navigation Top Stories Search. Generally I have found correlation between the two tests. Total and specific analysis of proteins in aastm goods The total quantity of latex proteins in gloves is evaluated by extracting the rubber with an aqueous solution and measuring according to ASTM D It is thought that proteins in the glove are being washed out and replaced by water molecules, creating diffusible channels through which small diameter species, like the HIV virus, could pass.

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Today, some 12, ASTM standards are used around the world to improve product quality, enhance safety, facilitate market access and trade, and build consumer confidence.

ASTM International – Standard References for ASTM D – 15

Potential hazards include skin absorption of harmful substances, chemical or thermal burns, electrical dangers, bruises, abrasions, cuts, punctures, fractures and amputations. It is reported that surgical gloves, long assumed effective barriers against blood-born pathogens, may not be effective as originally thought and, further, improperly manufactured gloves may cause a serious threat to healthcare workers and patients.

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About 10 percent of medical field employees dealing with dipped rubber products suffer from this allergy. A definitive procedure that produces a test result: The extracted protein is redissolved and quantified colorimetrically by the modified Lowry method using a protein standard. A precision and bias statement shall be reported at the end of a test method. The proteins then easily are removed. Two-sided coating is possible but expensive.

The fumed silica attaches itself to the rubber particle and substitutes the proteins. Latex processed without silica, does have protein residues, see Table II. Powder-free latex gloves and other methods of reducing protein content Latex gloves are among the most commonly used critical latex articles.

Login Register Subscribe Current Issue. It is suggested that the silica attaches itself to the surface of the latex particle substituting the protein molecules and displacing them, see Fig.

Hamilton, Journal of Allergy and Clinical Immunology, 96, Proteins in the latex migrate to the surface and penetrate through the skin. This problem has become more apparent in the last several years due to the increased use of rubber gloves for infectious diseases protection. It is assumed that all who use this test method will be trained analysts capable of performing common laboratory procedures skillfully and safely. Thompson et al9 that there is a relationship between hydration and compromised barriers in some surgical gloves.

The silica dipping process is simple, short and inexpensive. No items in cart. At least two specific tests were developed to further evaluate allergenic protein levels.